INCREASED SUPEROXIDE-DISMUTASE ACTIVITY IN AGED HUMAN CEREBROSPINAL-FLUID AND RAT-BRAIN DETERMINED BY ELECTRON-SPIN-RESONANCE SPECTROMETRY USING THE SPIN TRAP METHOD

被引：75

作者：

HIRAMATSU, M

KOHNO, M

EDAMATSU, R

MITSUTA, K

MORI, A

机构：

[1] OKAYAMA UNIV, SCH MED,INST NEUROBIOL,DEPT NEUROCHEM, 2-5-1 SHIKATA CHO, OKAYAMA 700, JAPAN

[2] YAMAGATA TECHNOPOLIS FDN, YAMAGATA, JAPAN

[3] JEOL LTD, TOKYO 196, JAPAN

来源：

JOURNAL OF NEUROCHEMISTRY | 1992年 / 58卷 / 03期

关键词：

SUPEROXIDE DISMUTASE ACTIVITY; HUMAN CSF; ELECTRON SPIN RESONANCE SPECTROMETRY; RAT BRAIN; AGING;

D O I：

10.1111/j.1471-4159.1992.tb09375.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Superoxide dismutase (SOD) activity in CSF of patients was determined by electron spin resonance spectrometry using the spin trap method. Variation in SOD activity was found among patients. SOD activity in CSF of subjects increased with age and this was identified as Cu,Zn-SOD activity by electrophoresis. In addition, animal experiments showed that SOD activities were higher in mitochondrial and cytosol fractions of aged rats than in those of adult rats. This finding on aged rat brain validates the increase of SOD activity in aged human CSF.

引用

页码：1160 / 1164

页数：5

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