ORGANIC-PHASE ENZYME ELECTRODE OPERATED IN WATER-FREE SOLVENTS

被引：64

作者：

DONG, SJ

GUO, YZ

机构：

[1] Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 22期

关键词：

D O I：

10.1021/ac00094a009

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new material, polyhydroxyl cellulose, and a refrigerating immobilization method were used to construct HRP-mediator electrode for determination of hydrogen peroxide in water-free organic solvents. Rapid and sensitive response was obtained. The enzyme electrode had a prolonged Lifetime of at least 2 months. A stable and high catalytic activity could be retained for the enzyme immobilized in this way. The material and immobilization described offer a new and effective way for enzyme stabilization in biosensor fabrication, especially for organic phase enzyme electrodes, which suffer a more hostile and harsh environment.

引用

页码：3895 / 3899

页数：5

共 15 条

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