SYNTENIC MAPPING AND CHROMOSOMAL LOCALIZATION OF BOVINE ALPHA-INTERFERON AND INTERFERON-BETA GENES

被引：18

作者：

RYAN, AM ^{[1
]}

GALLAGHER, DS ^{[1
]}

WOMACK, JE ^{[1
]}

机构：

[1] TEXAS A&M UNIV SYST,DEPT VET PATHOBIOL,COLL STN,TX 77843

来源：

MAMMALIAN GENOME | 1992年 / 3卷 / 10期

关键词：

D O I：

10.1007/BF00350624

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The previous assignment of bovine alpha-(IFNA) and beta-(IFNB) interferon gene families to syntenic group U18 was confirmed with additional cDNA probes and a bovine-rodent hybrid somatic cell panel representing all 29 bovine autosomal syntenic groups. Fluorescent in situ hybridization (FISH) localized these genes to bovine Chromosome (Chr) 8 band 15 and demonstrates that with biotinylated plasmids, as few as five tandemly arrayed sequences can be detected by conventional fluorescent microscopy. This technique can be applied to physical mapping of other multicopy genes in domestic animals.

引用

页码：575 / 578

页数：4

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