NUCLEOTIDE-SEQUENCE AND EXPRESSION IN ESCHERICHIA-COLI OF CDNAS ENCODING PAPAYA PROTEINASE OMEGA FROM CARICA-PAPAYA

被引：37

作者：

REVELL, DF ^{[1
]}

CUMMINGS, NJ ^{[1
]}

BAKER, KC ^{[1
]}

COLLINS, ME ^{[1
]}

TAYLOR, MAJ ^{[1
]}

SUMNER, IG ^{[1
]}

PICKERSGILL, RW ^{[1
]}

CONNERTON, IF ^{[1
]}

GOODENOUGH, PW ^{[1
]}

机构：

[1] INST FOOD RES, READING LAB, DEPT PROT ENGN, READING RG6 2EF, ENGLAND

来源：

GENE | 1993年 / 127卷 / 02期

关键词：

RECOMBINANT DNA; ZYMOGEN; ISOZYMES; CYSTEINE PROTEINASE;

D O I：

10.1016/0378-1119(93)90723-G

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have cloned and sequenced two similar, but distinct, cDNAs from both fruit and leaf tissues of Carica papaya. The C-terminal portion of the predicted amino acid (aa) sequence of one of the clones has complete identity with the mature enzyme sequence of the cysteine proteinase papaya proteinase omega (PpOMEGA). The second clone contains ten individual bp changes compared with the first and encodes a protein with three single-aa substitutions, only one of which is located in the mature sequence, but most noticeably carries an additional 19-aa C-terminal extension. The clones encode pre-pro precursor isoforms of PpOMEGA. The former of these clones has been expressed in Escherichia coli using a T7 polymerase expression system to produce insoluble pro-enzyme which has been solubilized and refolded to yield auto-activable pro-PpOMEGA.

引用

页码：221 / 225

页数：5

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