EXTRACTION AND ISOLATION OF MESSENGER-RNA FROM ADULT ARTICULAR-CARTILAGE

被引：58
作者：
ADAMS, ME
HUANG, DQ
YAO, LY
SANDELL, LJ
机构：
[1] UNIV CALGARY,JOINT INJURY RES GRP,CALGARY T2N 1N4,ALBERTA,CANADA
[2] UNIV WASHINGTON,DEPT ORTHOPAED SURG,SEATTLE,WA 98195
[3] UNIV WASHINGTON,DEPT BIOCHEM,SEATTLE,WA 98195
来源：
ANALYTICAL BIOCHEMISTRY | 1992年 / 202卷 / 01期
关键词：
D O I：
10.1016/0003-2697(92)90211-O
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a method to isolate RNA in high yield from adult articular cartilage. Homogenization of the articular cartilage with a freezer mill, extraction with 4 m guanidinium isothiocyanate/acid-phenol, and ultracentrifugation in cesium trifluoroacetate was found to be an effective and practical method for isolating a high yield of intact RNA from adult canine articular cartilage. The total RNA was suitable for Northern blot analysis. The mRNA that could then be isolated by oligo-dT affinity chromatography was found to be a suitable substrate for in vitro translation, for making a cDNA library, and for PCR amplification. © 1992.
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页码：89 / 95
页数：7
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