ISOLATION AND CHARACTERIZATION OF AN ALLERGEN FROM SHORT RAGWEED POLLEN

被引：83
作者：
UNDERDOWN, BJ
GOODFRIEND, L
机构：
[1] Medical Research Associate, Medical Research Council of Canada
来源：
BIOCHEMISTRY | 1969年 / 8卷 / 03期
关键词：
D O I：
10.1021/bi00831a031
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An allergenically active protein, antigen Ra.3, was isolated from the aqueous extract of short ragweed pollen by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography. Antigen Ra.3 was found to be highly purified on the basis of TEAE-cellulose chromatography, polyacrylamide disc electrophoresis, sedimentation velocity ultracentrifugation, gel filtration, and immunodiffusion tests. Antigen Ra.3 was shown to have a molecular weight of 15,000, an [formula omitted] a total hexose + pentose content of 12.4%, and an amino acid composition distinct from the major allergen of ragweed pollen, antigen E. by direct skin, passive sensitization, and cross-neutralization tests, antigens Ra.3 and E differed in allergenic specificity but shared common allergenic determinants. © 1969, American Chemical Society. All rights reserved.
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页码：980 / +
页数：1
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