SIMULTANEOUS ELECTROELUTION AND CONCENTRATION OF DNA FRAGMENTS FROM AGAROSE GELS

被引：2

作者：

STROOP, WG

机构：

[1] VET ADM MED CTR, NEUROVIROL RES LAB, SALT LAKE CITY, UT 84148 USA

[2] UNIV UTAH, SCH MED, DEPT NEUROL, SALT LAKE CITY, UT 84112 USA

[3] UNIV UTAH, SCH MED, DEPT PATHOL, SALT LAKE CITY, UT 84112 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1988年 / 169卷 / 01期

关键词：

D O I：

10.1016/0003-2697(88)90273-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and inexpensive method for the electroelution of DNA fragments from agarose gels is described. DNA fragments were separated by agarose gel electrophoresis and visualized by staining with ethidium bromide. Selected DNA fragments were placed into electroeluter tubes capped with dialysis membrane and electroeluted into a small volume of buffer using a conventional horizontal gel electrophoresis apparatus. The method successfully eluted and concentrated DNA fragments with molecular weights ranging from 2.7 to 13.9 MDa in 3 h.

引用

页码：194 / 196

页数：3

共 9 条

[1] BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[2] CLONING OF HERPES-SIMPLEX VIRUS TYPE-1 SEQUENCES REPRESENTING THE WHOLE GENOME
GOLDIN, AL
SANDRIGOLDIN, RM
LEVINE, M
GLORIOSO, JC
[J]. JOURNAL OF VIROLOGY, 1981, 38 (01) : 50 - 58
[3] MANIATIS T, 1982, MOL CLONING LABORATO, P150
[4] ANALYSIS OF RESTRICTION FRAGMENTS OF T7 DNA AND DETERMINATION OF MOLECULAR-WEIGHTS BY ELECTROPHORESIS IN NEUTRAL AND ALKALINE GELS
MCDONELL, MW
SIMON, MN
STUDIER, FW
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1977, 110 (01) : 119 - 146
[5] SCHLEIF RF, 1981, PRACTICAL METHODS MO, P90
[6] DETECTION OF 2 RESTRICTION ENDONUCLEASE ACTIVITIES IN HAEMOPHILUS-PARAINFLUENZAE USING ANALYTICAL AGAROSE-ETHIDIUM BROMIDE ELECTROPHORESIS
SHARP, PA
SUGDEN, B
SAMBROOK, J
[J]. BIOCHEMISTRY, 1973, 12 (16) : 3055 - 3063
[7] Smith H O, 1980, Methods Enzymol, V65, P371
[8] WU R, 1976, METHOD CANCER RES, V12, P87
[9] Yang R, 1979, Methods Enzymol, V68, P176

← 1 →