RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS OF A GLOBODERA-PALLIDA POPULATION SELECTED FOR VIRULENCE

被引：0

作者：

PASTRIK, KH ^{[1
]}

RUMPENHORST, HJ ^{[1
]}

BURGERMEISTER, W ^{[1
]}

机构：

[1] BIOL BUNDESANSTALT LAND & FORSTWIRTSCHAFT, INST NEMATOL & WIRBELTIERKUNDE, D-48161 MUNSTER, GERMANY

来源：

FUNDAMENTAL AND APPLIED NEMATOLOGY | 1995年 / 18卷 / 02期

关键词：

RAPD; DNA MARKER; VIRULENCE; SELECTION; GLOBODERA PALLIDA;

D O I：

暂无

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Selection of a Globodera pallida population on the partially resistant potato cultivar Darwina (Marijke x VTN2 62.33.3) for seven generations resulted in an increase of reproduction rate indicating a higher ratio of virulent genotppes in the selected population. Random amplified polymorphic DNA (RAPD) patterns of the selected and the unselected population were generally very similar with 40 primers tested. With two of these primers, however, two additional non-homologous DNA fragments were amplified in the selected population. RAPD analyses of single cysts revealed that the DNA properties leading to these amplified fragments were highly enriched in the selected population. One of the two DNA fragments was cloned and used to probe amplified DNA from sixteen G. pallida populations. Hybridization was only observed with populations showing higher reproduction rates on c.v. Darwina. This may indicate a correlation of the DNA fragment with a particular type of virulence.

引用

页码：109 / 114

页数：6

共 15 条

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