MATERNAL-SPECIFIC METHYLATION OF THE IMPRINTED MOUSE IGF2R LOCUS IDENTIFIES THE EXPRESSED LOCUS AS CARRYING THE IMPRINTING SIGNAL

被引：529

作者：

STOGER, R ^{[1
]}

KUBICKA, P ^{[1
]}

LIU, CG ^{[1
]}

KAFRI, T ^{[1
]}

RAZIN, A ^{[1
]}

CEDAR, H ^{[1
]}

BARLOW, DP ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,HADASSAH MED SCH,IL-91010 JERUSALEM,ISRAEL

来源：

CELL | 1993年 / 73卷 / 01期

关键词：

D O I：

10.1016/0092-8674(93)90160-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mouse insulin-like growth factor type 2 receptor (Igf2r) is imprinted and expressed exclusively from the maternally inherited chromosome. To investigate whether methylation could function as the imprinting signal, we have cloned 130 kb from the Igf2r locus and searched for sequences methylated in a parental-specific manner. Two regions have been identified: region 1 contains the start of transcription and is methylated only on the silent paternal chromosome; region 2 is contained in an intron and is methylated only on the expressed maternal chromosome. Methylation of region 1 is acquired after fertilization, in contrast with the methylation of region 2, which is inherited from the female gamete. Methylation of region 2 may mark the maternal Igf2r locus in a manner that could act as an imprinting signal. These data suggest that the expressed locus carries a potential imprinting signal and imply that methylation is necessary for expression of the Igf2r gene.

引用

页码：61 / 71

页数：11

共 39 条

[1]

ARTZT K, 1991, GENOME, V1, pS281

[2] THE MOUSE INSULIN-LIKE GROWTH-FACTOR TYPE-2 RECEPTOR IS IMPRINTED AND CLOSELY LINKED TO THE TME LOCUS [J].

BARLOW, DP ;

STOGER, R ;

HERRMANN, BG ;

SAITO, K ;

SCHWEIFER, N .

NATURE, 1991, 349 (6304) :84-87

[3] PARENTAL IMPRINTING OF THE MOUSE H19 GENE [J].

BARTOLOMEI, MS ;

ZEMEL, S ;

TILGHMAN, SM .

NATURE, 1991, 351 (6322) :153-155

[4]

BEDDINGTON RSP, 1989, DEVELOPMENT, V105, P733

[5]

BEECHEY CV, 1990, MOUSE GENOME, V87, P64

[6] DNA METHYLATION - EVOLUTION OF A BACTERIAL IMMUNE FUNCTION INTO A REGULATOR OF GENE-EXPRESSION AND GENOME STRUCTURE IN HIGHER EUKARYOTES [J].

BESTOR, TH .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1990, 326 (1235) :179-187

[7] THE ESSENTIALS OF DNA METHYLATION [J].

BIRD, A .

CELL, 1992, 70 (01) :5-8

[8] CPG-RICH ISLANDS AND THE FUNCTION OF DNA METHYLATION [J].

BIRD, AP .

NATURE, 1986, 321 (6067) :209-213

[9] BETA+ THALASSEMIA - ABERRANT SPLICING RESULTS FROM A SINGLE POINT MUTATION IN AN INTRON [J].

BUSSLINGER, M ;

MOSCHONAS, N ;

FLAVELL, RA .

CELL, 1981, 27 (02) :289-298

[10] PARENTAL-SPECIFIC METHYLATION OF AN IMPRINTED TRANSGENE IS ESTABLISHED DURING GAMETOGENESIS AND PROGRESSIVELY CHANGES DURING EMBRYOGENESIS [J].

CHAILLET, JR ;

VOGT, TF ;

BEIER, DR ;

LEDER, P .

CELL, 1991, 66 (01) :77-83

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