SEPARATION OF LARGE DNA-MOLECULES FROM PLEUROTUS-CORNUCOPIAE BY PULSED-FIELD GEL-ELECTROPHORESIS

被引：0

作者：

TAMAI, Y ^{[1
]}

YOKONO, T ^{[1
]}

SUNAGAWA, M ^{[1
]}

KOJIMA, Y ^{[1
]}

MIURA, K ^{[1
]}

机构：

[1] M&S INSTRUMENTS TRADING INC, OSAKA 532, JAPAN

来源：

MOKUZAI GAKKAISHI | 1995年 / 41卷 / 10期

关键词：

PULSED-FIELD GEL ELECTROPHORESIS; PFGE; PLEUROTUS CORNUCOPIAE; CHEF; ELECTROPHORETIC KARYOTYPE;

D O I：

暂无

中图分类号：

TB3 [工程材料学]; TS [轻工业、手工业、生活服务业];

学科分类号：

0805 ; 080502 ; 0822 ;

摘要：

An optimum condition for the separation of large DNA molecules from Pleurotus cornucopiae (Paulet) Rolland var. citrinopileatus (Sing.) Ohira was investigated by using the pulsed-field gel electrophoresis (PFGE) method. A clear separation of the DNA molecules was achieved by, a combination of switching intervals and running voltages. Nine and seven bands were separated from the dikaryon and the monokaryon, respectively, within 96 h. They ranged in size from 1.7 to 3.7 Mb. Their genome sizes were estimated as about 55.2 Mb and 27.8 Mb, respectively.

引用

页码：952 / 955

页数：4

共 14 条

[1] ELECTROPHORETIC KARYOTYPE OF ASPERGILLUS-NIDULANS [J].