CD44 VARIANT EXON EPITOPES IN PRIMARY BREAST-CANCER AND LENGTH OF SURVIVAL

被引:370
|
作者
KAUFMANN, M
HEIDER, KH
SINN, HP
VONMINCKWITZ, G
PONTA, H
HERRLICH, P
机构
[1] FORSCHUNGSZENTRUM KARLSRUHE,INST GENET,KARLSRUHE,GERMANY
[2] UNIV HEIDELBERG,DEPT PATHOL,D-69115 HEIDELBERG,GERMANY
来源
LANCET | 1995年 / 345卷 / 8950期
关键词
D O I
10.1016/S0140-6736(95)90521-9
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
CD44 designates a group of closely related cell-surface proteins generated by alternative splicing. We have previously shown that splice variants carrying sequences encoded by exon v6 are preferentially expressed in metastatic animal cancer cell lines and that they confer metastatic behaviour on non-metastatic animal tumour cell lines. In this study we set out to assess the expression of CD44 epitopes specific for variant exon sequences in human breast cancer and their potential for determining prognosis. We used affinity-purified polyclonal sera and four monoclonal antibodies raised against the human homologues of CD44 variant exon sequences to investigate the presence of CD44 on 100 primary invasive breast tumours, 12 local recurrences, 18 lymph node metastases, and normal tissue controls. Whereas normal mammary ductal epithelial cells and cells derived from hyperplastic lesions do not express CD44 variant exons, expression of v3, v5, and v6 epitopes was found in most tumour samples. The Dill (exon v6) epitope was present in 84% of the primary tumours and in 100% of axillary lymph node metastases and local recurrences. The presence of these CD44 epitopes is correlated with poor overall survival. 15 patients with exon-v6-negative tumours had good survival compared with 76 patients with exon-v6-positive tumours (p=0.005; log rank test). Multivariate analysis showed that the CD44 epitope encoded by exon v6 was a good marker for prognosis independent of progesterone receptor, lymph node status, tumour size, and grade.
引用
收藏
页码:615 / 619
页数:5
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