ALLOSTERIC CONTROL OF BIODEGRADATIVE L-THREONINE DEHYDRASE - EFFECT OF AMP ON AN EARLY STEP IN REACTION MECHANISM

被引：11
作者：
NIEDERMAN, RA
RABINOWITZ, KW
WOOD, WA
机构：
[1] Department of Biochemistry, Michigan State University, East Lansing
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 36卷 / 06期
关键词：
D O I：
10.1016/0006-291X(69)90296-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In highly purified L-threonine dehydrase preparations, allosteric activation by AMP was accompanied by: 1) a marked enhancement in the accumulation of an enzyme-substrate intermediary which absorbs at 455 mμ; 2) a marked increase in substrate-induced loss of circular dichroism at 415 mμ; 3) an increase in the affinity of the dehydrase for competitive inhibitors which are unable to undergo dehydration. These findings suggest that the effect of AMP is exerted during the first reactions of the overall dehydration process. © 1969.
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页码：951 / +
页数：1
相关论文
共 9 条
[1]   INTERACTIONS BETWEEN SUBUNITS OF TRYPTOPHAN SYNTHETASE OF ESCHERICHIA COLI . OPTICAL PROPERTITES OF AN INTERMEDIATE BOUND TO ALPHA2BETA2 COMPLEX [J].
GOLDBERG, ME ;
BALDWIN, RL .
BIOCHEMISTRY, 1967, 6 (07) :2113-&
[2]   COLUMN CHROMATOGRAPHY OF PROTEINS - CALCIUM PHOSPHATE [J].
LEVIN, O .
METHODS IN ENZYMOLOGY, 1962, 5 :27-32
[3]   CIRCULAR DICHROISM OF BIODEGRADATIVE THREONINE DEAMINASE [J].
NAKAZAWA, A ;
TOKUSHIGE, M ;
HAYAISHI, O .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1967, 29 (02) :184-+
[4]  
PHILLIPS AT, 1964, BIOCHEM BIOPH RES CO, V16, P530
[5]  
PHILLIPS AT, 1965, J BIOL CHEM, V240, P4703
[6]   ON ROLE OF PYRIDOXAL PHOSPHATE FOR AMP-DEPENDENT THREONINE DEAMINASE OF ESCHERICHIA COLI [J].
TOKUSHIGE, M .
JOURNAL OF VITAMINOLOGY, 1967, 13 (02) :165-+
[7]  
TOKUSHIGE M, 1968, S PYRIDOXAL ENZYMES, P105
[8]  
WHANGER PD, 1968, J BIOL CHEM, V243, P167
[9]  
WOOD WA, 1949, J BIOL CHEM, V181, P171
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