Lipase activity in the milk and blood of four cows was measured during their lactation. Peak blood plasma lipase values occurred about 24 hr before observed estrus. These peak values were bracketed by lower values occurring two or three days before and one to three days after the day of observed heat. Changes in blood lipase activity were reflected and magnified in the milk, although these changes occurred 9 to 15 hr after they were observed in the blood. © 1969, American Dairy Science Association. All rights reserved.