COMPARATIVE QUALITY-CONTROL OF SOME BONE-SEEKING TC-99M RADIOPHARMACEUTICALS BY GEL CHROMATOGRAPHY

The radiochemical purity of the three osteopatic ligands: Tc-99m(Sn)-PyP, Tc-99m(Sn)-DPD and Tc-99m(Sn)-MDP has been determined by gel chromatography on Sephadex. The results of the analyses strongly depend on the composition of the eluent. The dilution effect of pure saline as eluent was observed in all the preparations examined. The most sensitive was found to be Tc-99m(Sn);PyP. The retention of Tc-99m activity bound to the gel matrix (Tc-99m-hydrolyte) was over 30%. The diphosphonates were found to be more stable (retention 10-15%). The retention is substantially lower, i.e. a high recovery of the labeled complexes is obtained when the eluent contains the ligand. The best results are obtained when the eluent contains the same concentrations of ligand and reductant as in the labeled complex. There was no significant difference in the behavior of the given radiopharmaceuticals prepared as a fresh solution and in the freeze-dried kit.