ALTERNATIVE SPLICING OF HUMAN G6PD MESSENGER-RNA IN K562 CELLS BUT NOT IN CULTURED ERYTHROBLASTS

被引：1

作者：

CAPPELLINI, MD ^{[1
]}

TAVAZZI, D ^{[1
]}

DIMONTEMUROS, FM ^{[1
]}

SAMPIETRO, M ^{[1
]}

GAVIRAGHI, A ^{[1
]}

CARANDINI, D ^{[1
]}

FIORELLI, G ^{[1
]}

机构：

[1] UNIV MILAN,OSPED MAGGIORE POLICLIN,IST MED INTERNA & FISIOPATOL MED,PAD LITTA,I-20122 MILAN,ITALY

来源：

EUROPEAN JOURNAL OF CLINICAL INVESTIGATION | 1993年 / 23卷 / 03期

关键词：

ALTERNATIVE SPLICING; G6PD MESSENGER RNA ERYTHROBLASTS;

D O I：

10.1111/j.1365-2362.1993.tb00760.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The biochemical properties of glucose-6-phosphate dehydrogenase (G6PD) vary in different tissues, and different protein isoforms of the enzyme have been described. Alternative splicing of G6PD intron VII has been detected in transformed lymphoblasts, granulocytes and spermatocytes; the function of this mRNA species is still unknown. We developed a PCR for detecting alternatively spliced G6PD mRNA in K562 and in erythroblasts at different stage of maturation obtained from human peripheral BFU-E in order to evaluate a possible physiological role during erythroid maturation. Trace events of alternative splicing of G6PD intron VII sequences were observed in K562 cells but not in BFU-E-derived erythroid precursors, we consider this phenoemenon a non-functional activity in the cells analysed.

引用

页码：188 / 191

页数：4

共 15 条

[1] ALTERNATIVE SPLICING - A UBIQUITOUS MECHANISM FOR THE GENERATION OF MULTIPLE PROTEIN ISOFORMS FROM SINGLE GENES [J].