SALT HYDRATES BUFFER WATER ACTIVITY DURING CHYMOTRYPSIN-CATALYZED PEPTIDE-SYNTHESIS

被引：50
作者：
KUHL, P
HALLING, PJ
机构：
[1] UNIV STRATHCLYDE,DEPT BIOSCI & BIOTECHNOL,GLASGOW G1 1XW,SCOTLAND
[2] KARL MARX UNIV,SEKT BIOWISSENSCH,O-7010 LEIPZIG,GERMANY
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1078卷 / 03期
关键词：
ORGANIC MEDIA; SALT HYDRATE; WATER ACTIVITY; PEPTIDE SYNTHESIS;
D O I：
10.1016/0167-4838(91)90151-O
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Chymotrypsin (EC 3.4.21.1) powder suspended in hexane in the presence of Na2CO3.10H2O is a good catalyst for peptide synthesis. The salt hydrate releases water to fix the thermodynamic water activity of the system in accord with its dissociation pressure. Salt hydrates can be useful to buffer water activity in mainly organic enzyme reaction mixtures at a value permitting activity of the catalyst while minimising hydrolytic side reactions.
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收藏
页码：326 / 328
页数：3
相关论文
共 11 条
[1]   AGAROSE-CHYMOTRYPSIN AS A CATALYST FOR PEPTIDE AND AMINO-ACID ESTER SYNTHESIS IN ORGANIC MEDIA [J].
BLANCO, RM ;
GUISAN, JM ;
HALLING, PJ .
BIOTECHNOLOGY LETTERS, 1989, 11 (11) :811-816
[2]   ORGANIC LIQUIDS AND BIOCATALYSTS - THEORY AND PRACTICE [J].
HALLING, P .
TRENDS IN BIOTECHNOLOGY, 1989, 7 (03) :50-52
[3]   HIGH-AFFINITY BINDING OF WATER BY PROTEINS IS SIMILAR IN AIR AND IN ORGANIC-SOLVENTS [J].
HALLING, PJ .
BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1040 (02) :225-228
[4]   EFFECTS OF WATER ON EQUILIBRIA CATALYZED BY HYDROLYTIC ENZYMES IN BIPHASIC REACTION SYSTEMS [J].
HALLING, PJ .
ENZYME AND MICROBIAL TECHNOLOGY, 1984, 6 (11) :513-516
[5]   MEASUREMENT AND CONTROL OF WATER ACTIVITY WITH AN ALUMINUM-OXIDE SENSOR IN ORGANIC 2-PHASE REACTION MIXTURES FOR ENZYMATIC CATALYSIS [J].
KHAN, SA ;
HALLING, PJ ;
BELL, G .
ENZYME AND MICROBIAL TECHNOLOGY, 1990, 12 (06) :453-458
[6]  
KHAN SA, 1989, THESIS U STRATHCLYDE
[7]  
KUHL P, 1981, PHARMAZIE, V36, P463
[8]   CHYMOTRYPSIN SUSPENDED IN ORGANIC-SOLVENTS WITH SALT HYDRATES IS A GOOD CATALYST FOR PEPTIDE-SYNTHESIS FROM MAINLY UNDISSOLVED REACTANTS [J].
KUHL, P ;
HALLING, PJ ;
JAKUBKE, HD .
TETRAHEDRON LETTERS, 1990, 31 (36) :5213-5216
[9]  
ZAKS A, 1988, J BIOL CHEM, V263, P3194
[10]  
ZAKS A, 1988, J BIOL CHEM, V263, P8017
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