MICROHETEROGENEITY OF CARBOHYDRATE GROUP OF ASPERGILLUS ORYZAE ALPHA-AMYLASE

被引：251

作者：

MCKELVY, JF

LEE, YC

机构：

[1] Department of Biology, McCollum-Pratt Institute, The Johns Hopkins University, Baltimore

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 132卷 / 01期

关键词：

D O I：

10.1016/0003-9861(69)90341-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

α-Amylase from Aspergillus oryzae was purified by gel filtration and ion-exchange chromatography. It appeared to be homogeneous by ultracentrifugation, electrophoresis, and ion-exchange chromatography, and contained glucosamine, mannose, galactose, arabinose, and xylose. Exhaustive proteolysis of the amylase yielded Ser · Asp(NH · Oligosaccharide) and Asp(NH · Oligosaccharide). By chromatography on Dowex 50-X2, the latter glycopeptide could be fractionated into several peaks, each having a different sugar composition. This implies a heterogeneity of the carbohydrate group of the α-amylase. © 1969.

引用

页码：99 / &

共 29 条

[1] STRUCTURE OF GLYCOPEPTIDE OBTAINED FROM TAKA-AMYLASE A [J].