MICROHETEROGENEITY OF CARBOHYDRATE GROUP OF ASPERGILLUS ORYZAE ALPHA-AMYLASE

被引：250
作者：
MCKELVY, JF
LEE, YC
机构：
[1] Department of Biology, McCollum-Pratt Institute, The Johns Hopkins University, Baltimore
来源：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 132卷 / 01期
关键词：
D O I：
10.1016/0003-9861(69)90341-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
α-Amylase from Aspergillus oryzae was purified by gel filtration and ion-exchange chromatography. It appeared to be homogeneous by ultracentrifugation, electrophoresis, and ion-exchange chromatography, and contained glucosamine, mannose, galactose, arabinose, and xylose. Exhaustive proteolysis of the amylase yielded Ser · Asp(NH · Oligosaccharide) and Asp(NH · Oligosaccharide). By chromatography on Dowex 50-X2, the latter glycopeptide could be fractionated into several peaks, each having a different sugar composition. This implies a heterogeneity of the carbohydrate group of the α-amylase. © 1969.
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页码：99 / &
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