IMPROVED PULSE SEQUENCES FOR MEASURING COUPLING-CONSTANTS IN C-13,N-15-LABELED PROTEINS

被引：0

作者：

MADSEN, JC

SORENSEN, OW

SORENSEN, P

POULSEN, FM

机构：

[1] NOVO NORDISK AS,DK-2880 BAGSVAERD,DENMARK

[2] CARLSBERG LAB,KEM AFDELING,DK-2500 VALBY,DENMARK

来源：

JOURNAL OF BIOMOLECULAR NMR | 1993年 / 3卷 / 02期

关键词：

MULTIDIMENSIONAL NMR SPECTROSCOPY; C-13; N-15-LABELED PROTEIN; COUPLING CONSTANT;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NMR pulse sequences for measuring coupling constants in C-13,N-15-labeled proteins are presented. These pulse sequences represent improvements over earlier experiments with respect to resolution and number of radiofrequency pulses. The experiments are useful for measuring J(NH)beta, J(NCO), J(NC)alpha, J(H)N(CO) and J(H)N(H)alpha. Applications to chymotrypsin inhibitor 2 (CI-2) are shown.

引用

页码：239 / 244

页数：6

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