IMPROVED PULSE SEQUENCES FOR MEASURING COUPLING-CONSTANTS IN C-13,N-15-LABELED PROTEINS

被引：0

作者：

MADSEN, JC

SORENSEN, OW

SORENSEN, P

POULSEN, FM

机构：

[1] NOVO NORDISK AS,DK-2880 BAGSVAERD,DENMARK

[2] CARLSBERG LAB,KEM AFDELING,DK-2500 VALBY,DENMARK

来源：

JOURNAL OF BIOMOLECULAR NMR | 1993年 / 3卷 / 02期

关键词：

MULTIDIMENSIONAL NMR SPECTROSCOPY; C-13; N-15-LABELED PROTEIN; COUPLING CONSTANT;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NMR pulse sequences for measuring coupling constants in C-13,N-15-labeled proteins are presented. These pulse sequences represent improvements over earlier experiments with respect to resolution and number of radiofrequency pulses. The experiments are useful for measuring J(NH)beta, J(NCO), J(NC)alpha, J(H)N(CO) and J(H)N(H)alpha. Applications to chymotrypsin inhibitor 2 (CI-2) are shown.

引用

页码：239 / 244

页数：6

共 50 条

[1] DETERMINATION OF H(N),H-ALPHA AND H(N), C' COUPLING-CONSTANTS IN C-13,N-15-LABELED PROTEINS
WEISEMANN, R
RUTERJANS, H
SCHWALBE, H
SCHLEUCHER, J
BERMEL, W
GRIESINGER, C
JOURNAL OF BIOMOLECULAR NMR, 1994, 4 (02) : 231 - 240
[2] NEW METHODS FOR THE MEASUREMENT OF NH-C-ALPHA-H COUPLING-CONSTANTS IN N-15-LABELED PROTEINS
KAY, LE
BAX, A
JOURNAL OF MAGNETIC RESONANCE, 1990, 86 (01): : 110 - 126
[3] VICINAL C-13 N-15 COUPLING-CONSTANTS IN PEPTIDES
DIBLASI, R
KOPPLE, KD
JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1975, (02) : 33 - 34
[4] N-15C-13 COUPLING-CONSTANTS IN N-15 LABELED AZAADAMANTANE
BERGER, S
KALETSCH, H
ORGANIC MAGNETIC RESONANCE, 1976, 8 (08): : 438 - 438
[5] Determination of homo- and heteronuclear coupling constants in uniformly C-13,N-15-labeled DNA oligonucleotides
Zimmer, DP
Marino, JP
Griesinger, C
MAGNETIC RESONANCE IN CHEMISTRY, 1996, 34 : S177 - S186
[6] C-13 CHEMICAL-SHIFTS AND C-13-N-15-COUPLING CONSTANTS OF SOME N-15-LABELED BRIDGEHEAD NITRO-COMPOUNDS
DELLA, EW
JANOWSKI, WK
KASUM, B
KIRKBRIDE, KP
SHIRLEY, NJ
HETEROATOM CHEMISTRY, 1992, 3 (01) : 33 - 36
[7] RELATIONSHIP BETWEEN N-15 C-13 COUPLING-CONSTANTS AND HYBRIDIZATION
SCHULMAN, JM
VENANZI, T
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1976, 98 (21) : 6739 - 6741
[8] C-13 NMR - THE STEREOCHEMICAL DEPENDENCE OF N-15-C-13 COUPLING-CONSTANTS IN PYRAZOLES
AXENROD, T
MANGIARACINA, P
BULUSU, S
WATNICK, CM
WIEDER, MJ
ORGANIC MAGNETIC RESONANCE, 1980, 13 (03): : 197 - 199
[9] C-13,C-13 COUPLING-CONSTANTS OF CONJUGATED DIENES
STOCKER, M
KLESSINGER, M
WILHELM, K
ORGANIC MAGNETIC RESONANCE, 1981, 17 (02): : 153 - 155
[10] CONFORMATIONAL DEPENDENCE OF N-15(C-13) SPIN SPIN COUPLING-CONSTANTS
BERGER, S
TETRAHEDRON, 1978, 34 (20) : 3133 - 3136

← 1 2 3 4 5 →