YAC MAPPING BY FISH USING ALU-PCR-GENERATED PROBES

被引：53

作者：

BREEN, M ^{[1
]}

ARVEILER, B ^{[1
]}

MURRAY, I ^{[1
]}

GOSDEN, JR ^{[1
]}

PORTEOUS, DJ ^{[1
]}

机构：

[1] UNIV EDINBURGH,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND

来源：

GENOMICS | 1992年 / 13卷 / 03期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0888-7543(92)90148-L

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Human genomic mapping has been greatly advanced by the independent development of three new methods: large DNA fragment cloning in yeast artificial chromosomes, amplification from complex DNAs of human specific segments by Alu-PCR, and high-resolution localization of complex DNA probes by fluorescent in situ hybridization. We describe here the combination of these three analytical tools for efficient and accurate localization of randomly screened or especially selected human YAC recombinants to chromosome 11. We map a YAC clone encompassing the pepsinogen A (PGA) locus to 11q13.1-11q13.3. © 1992.

引用

收藏

页码：726 / 730

页数：5

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