IDENTIFICATION AND CLONING OF A MEGAKARYOCYTE GROWTH AND DEVELOPMENT FACTOR THAT IS A LIGAND FOR THE CYTOKINE RECEPTOR MPL

被引：869
作者：
BARTLEY, TD
BOGENBERGER, J
HUNT, P
LI, YS
LU, HS
MARTIN, F
CHANG, MS
SAMAL, B
NICHOL, JL
SWIFT, S
JOHNSON, MJ
HSU, RY
PARKER, VP
SUGGS, S
SKRINE, JD
MEREWETHER, LA
CLOGSTON, C
HSU, E
HOKOM, MM
HORNKOHL, A
CHOI, E
PANGELINAN, M
SUN, Y
MAR, V
MCNINCH, J
SIMONET, L
JACOBSEN, F
XIE, C
SHUTTER, J
CHUTE, H
BASU, R
SELANDER, L
TROLLINGER, D
SIEU, L
PADILLA, D
TRAIL, G
ELLIOTT, G
IZUMI, R
COVEY, T
CROUSE, J
GARCIA, A
XU, W
DELCASTILLO, J
BIRON, J
COLE, S
HU, MCT
PACIFICI, R
PONTING, I
SARIS, C
WEN, D
机构：
[1] Amgen, Incorporated Amgen Center Thousand Oaks
来源：
CELL | 1994年 / 77卷 / 07期
关键词：
D O I：
10.1016/0092-8674(94)90450-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel megakaryocyte growth and development factor (MGDF) has been identified in aplastic canine plasma, and its cDNAs have been cloned from canine, murine, and human sources. Purified canine MGDF isolated by procedures involving Mpl receptor affinity chromatography exists in at least two forms, with apparent molecular masses of 25 kDa and 31 kDa, that share the N-terminal amino acid sequence APPACDPRLLNKMLRDSHVLH. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. Canine MGDF and recombinant human MGDF support the development of megakaryocytes from human CD34(+) progenitor cell populations in liquid culture and promote the survival of a factor-dependent murine cell line (32D) engineered to express Mpl. These biological activities are blocked by the soluble extracellular domain of Mpl. These data demonstrate that MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPI receptor.
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页码：1117 / 1124
页数：8
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