A GENOMIC LIBRARY OF LYCOPERSICON PENNELLII IN LYCOPERSICON-ESCULENTUM - A TOOL FOR FINE MAPPING OF GENES

被引：255

作者：

ESHED, Y ^{[1
]}

ZAMIR, D ^{[1
]}

机构：

[1] OTTO WARBURG CTR BIOTECHNOL,IL-76100 REHOVOT,ISRAEL

来源：

EUPHYTICA | 1994年 / 79卷 / 03期

关键词：

GENE MAPPING; GENOMIC LIBRARY; LYCOPERSICON; RFLP; WILD GERMPLASM;

D O I：

10.1007/BF00022516

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The cultivated tomato contains only a small fraction of the genetic variation present in its wild relatives. In order to use the wild germplasm in tomato breeding and genetic studies we developed a new kind of genetic resource which is composed of 50 L. esculentum lines each containing a single introgression from the green fruited species L. pennellii (LA 716). Each of the introgression lines is nearly isogenic to the cultivated tomato; these lines provide complete coverage of the wild species genome. The lines contain on the average an introgression of 33 cM from a total genome size of 1200 cM. The size and identity of the introgressed segments was determined based on RFLP analysis of 350 markers. This resource can be viewed as a genomic library of the wild species in the cultivated background. It covers the entire genome with single independent 'inserts' per line and therefore every phenotypic difference between the introgression lines can be associated with the unique introgressed segment. The development and potential application of this resource are discussed.

引用

页码：175 / 179

页数：5

共 8 条

[1] Lycopersicon esculentum lines containing small overlapping introgressions from L. pennellii [J].