TRANSLESIONAL SYNTHESIS ON A DNA-TEMPLATE CONTAINING A SINGLE STEREOISOMER OF DG-(+)ANTI-BPDE OR DG-(-)-ANTI-BPDE (7,8-DIHYDROXY-ANTI-9,10-EPOXY-7,8,9,10-TETRAHYDROBENZO[A]PYRENE)

被引:117
作者
SHIBUTANI, S [1 ]
MARGULIS, LA [1 ]
GEACINTOV, NE [1 ]
GROLLMAN, AP [1 ]
机构
[1] NYU, DEPT CHEM, NEW YORK, NY 10003 USA
关键词
D O I
10.1021/bi00080a027
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Oligodeoxynucleotides modified site-specificially with dG-(+)-trans- and dG-(+)-cis-anti-BPDE (7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene) or dG-(-)-trans- and dG-(-)-cis-anti-BPDE were used as templates in primer extension reactions catalyzed by the Klenow fragment of Escherichia coli DNA polymerase I. The primer could be extended past the dG-(-)-trans-BPDE adduct with small amounts of dAMP incorporated opposite the lesion. A small amount of base deletions was also observed while, with the dG-(-)-cis-BPDE adduct, one- and two-base deletions predominated. When templates containing dG-(+)-trans-BPDE were used, small amounts of products containing one-base deletions were observed; with dG-(+)-cis-BPDE, substitution of dAMP opposite the lesion was also detected. The frequency of nucleotide insertion for dAMP opposite dG-(-)-trans-BPDE and the frequency of extension from the primer terminus containing the dA:dG-(-)-trans-BPDE pair were much higher than those observed with the other, stereochemically different BPDE adducts. Kinetic studies were in agreement with the results of the primer extension study. When the base flanking the 5' side of dG-BPDE was changed from dC to dT, the frequency of one-base deletions increased. We conclude that the trans- or cis-addition product of dG-(-)-anti-BPDE has a higher miscoding potential than dG-(+)-anti-BPDE in our model system and that G-->T transversions and deletions predominate. These observations are consistent with the types of mutations observed in vivo.
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页码:7531 / 7541
页数:11
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