A SINGLE-STRANDED-DNA EXONUCLEASE FROM SCHIZOSACCHAROMYCES-POMBE

被引：20
|
作者：
SZANKASI, P [1 ]
SMITH, GR [1 ]
机构：
[1] FRED HUTCHINSON CANC RES CTR,1124 COLUMBIA ST,SEATTLE,WA 98104
来源：
BIOCHEMISTRY | 1992年 / 31卷 / 29期
关键词：
D O I：
10.1021/bi00144a017
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have purified to near homogeneity a DNA exonuclease from meiotic cells of Schizosaccharomyces pombe. The enzyme, designated exonuclease II (ExoII), had an apparent molecular weight of 134 000 and was abundant in the cell. It specifically degraded single-stranded DNA in the 5'--> 3' direction with an apparent K(m) for 5' DNA ends of 3.6 X 10(-11) M and produced 5' deoxynucleoside monophosphates. Its mode of degradation is similar to that of the RecJ protein from Escherichia coli; ExoII may, therefore, be involved in genetic recombination and DNA damage repair.
引用
收藏
页码：6769 / 6773
页数：5
相关论文
共 50 条
  • [31] TRANSFORMATION OF SCHIZOSACCHAROMYCES-POMBE BY ELECTROPORATION
    HOOD, MT
    STACHOW, C
    NUCLEIC ACIDS RESEARCH, 1990, 18 (03) : 688 - 688
  • [32] THE YPT PROTEINS OF SCHIZOSACCHAROMYCES-POMBE
    ARMSTRONG, J
    PIDOUX, A
    BOWDEN, S
    CRAIGHEAD, M
    BONE, N
    ROBINSON, E
    BIOCHEMICAL SOCIETY TRANSACTIONS, 1994, 22 (02) : 460 - 463
  • [33] PREPARATION OF SPHEROPLASTS OF SCHIZOSACCHAROMYCES-POMBE
    DICKINSON, DP
    ISENBERG, I
    JOURNAL OF GENERAL MICROBIOLOGY, 1982, 128 (MAR): : 651 - 654
  • [34] MALATE DEHYDROGENASES IN SCHIZOSACCHAROMYCES-POMBE
    FLURY, U
    FIECHTER, A
    PATHOLOGIA ET MICROBIOLOGIA, 1974, 40 (3-4): : 145 - 146
  • [35] MALATE TRANSPORT IN SCHIZOSACCHAROMYCES-POMBE
    OSOTHSILP, C
    SUBDEN, RE
    JOURNAL OF BACTERIOLOGY, 1986, 168 (03) : 1439 - 1443
  • [36] IDENTIFICATION AND ISOLATION OF CORE HISTONES FROM SCHIZOSACCHAROMYCES-POMBE
    WHITESIDE, SG
    PLOCKE, DJ
    FEBS LETTERS, 1988, 226 (02) : 250 - 254
  • [37] SPECIFIC INITIATION AT AN ORIGIN OF REPLICATION FROM SCHIZOSACCHAROMYCES-POMBE
    CADDLE, MS
    CALOS, MP
    MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (03) : 1796 - 1805
  • [38] PROPERTIES OF PYRUVATE-KINASE FROM SCHIZOSACCHAROMYCES-POMBE
    DUNCAN, D
    MCGOWAN, J
    PRICE, NC
    BIOCHEMICAL SOCIETY TRANSACTIONS, 1989, 17 (03) : 560 - 561
  • [39] GROWTH OF SINGLE CELLS OF SCHIZOSACCHAROMYCES-POMBE UNDER NUTRIENT LIMITATION
    KOTHARI, IR
    REILLY, PJ
    MARTIN, PJ
    EAKMAN, JM
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1971, (NSEP): : 18 - &
  • [40] THE CLONING AND CHARACTERIZATION OF A RAS GENE FROM SCHIZOSACCHAROMYCES-POMBE
    NADINDAVIS, SA
    YANG, RCA
    NARANG, SA
    NASIM, A
    JOURNAL OF MOLECULAR EVOLUTION, 1986, 23 (01) : 41 - 51
12345