INTEGRIN-ALPHA-2-BETA-1 (VLA-2) MEDIATES REORGANIZATION AND CONTRACTION OF COLLAGEN MATRICES BY HUMAN-CELLS

被引：354
作者：
SCHIRO, JA
CHAN, BMC
ROSWIT, WT
KASSNER, PD
PENTLAND, AP
HEMLER, ME
EISEN, AZ
KUPPER, TS
机构：
[1] WASHINGTON UNIV,SCH MED,DEPT PATHOL,ST LOUIS,MO 63110
[2] HARVARD UNIV,SCH MED,DANA FARBER CANC INST,BOSTON,MA 02115
来源：
CELL | 1991年 / 67卷 / 02期
关键词：
D O I：
10.1016/0092-8674(91)90191-Z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The capacity of cells to organize and contract collagen fibrils is fundamental to processes as diverse as embryogenesis and wound healing. We analyzed different beta-1 integrins on diploid fibroblasts for their role in modifying the tertiary structure of collagen matrices. Using monoclonal antibodies that block the interaction of integrins with their ligands, evidence was obtained that alpha-2-beta-1 integrin is required for the contraction of a type I collagen matrix. Further supporting the role of alpha-2-beta-1, cell lines expressing minimal levels of this integrin uniformly failed to contract collagen matrices. In addition, transfection of a full-length alpha-2 cDNA into one such cell line led to enhanced cell surface expression of alpha-2-beta-1 and conferred the de novo capacity to contract collagen matrices.
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页码：403 / 410
页数：8
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