MICELLE-ENHANCED LUCIGENIN CHEMILUMINESCENCE FOR THE DETERMINATION OF CATECHOLAMINES

Aqueous solutions of cationic micellar hexadecyltrimethylammonium hydroxide (CTAOH) and nonionic micellar polyoxyethylene(23) dodecanol enhanced the intensity of maximum chemiluminescence (CL) light emission from the reaction of 10,10'-dimethyl-9,9'-biacridinium dinitrate (lucigenin) with catecholamines. In contrast, a lowering of the CL emission was observed in the presence of anionic micellar sodium dodecylsulfate. The best sensitivity was achieved in micellar CTAOH: logarithmic calibration curves of adrenaline, dopamine and noradrenaline were linear over the range from the detection limit of 1×10-8, 3×10-7 and 5×10-7 M up to 1×10-4 M, respectively. In the presence of micellar CTAOH, improvements in the sensitivity by factors of 1.6-10 were observed relative to that in water. The relative standard deviation was 2.5% in the micellar media. © 1990, The Japan Society for Analytical Chemistry. All rights reserved.