A HIGHLY SENSITIVE ASSAY FOR ADENOSINE-TRIPHOSPHATE EMPLOYING AN IMPROVED FIREFLY LUCIFERASE REAGENT

被引：5
|
作者：
SIMPSON, WJ
FERNANDEZ, JL
HAMMOND, JRM
SENIOR, PS
MCCARTHY, BJ
JAGO, PH
SIDOROWICZ, S
JASSIM, SAA
DENYER, SP
机构：
[1] BRITISH TEXT TECHNOL GRP,LEEDS LS16 6QL,ENGLAND
[2] WATER RES CTR,MARLOW SL7 2HD,BUCKS,ENGLAND
[3] UNIV NOTTINGHAM,DEPT PHARMACEUT SCI,NOTTINGHAM NG7 2RD,ENGLAND
来源：
LETTERS IN APPLIED MICROBIOLOGY | 1990年 / 11卷 / 04期
关键词：
D O I：
10.1111/j.1472-765X.1990.tb00162.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An improved firefly luciferase reagent allowed reliable detection of as little as 80 fmo1/1 adenosine triphosphate (ATP). Contamination by ATP of the deionized water diluent limited the sensitivity of the assay in some laboratories. The reagent can be used to measure ATP from less than ten bacterial cells if care is taken to eliminate ATP from equipment and reagents. Copyright © 1990, Wiley Blackwell. All rights reserved
引用
收藏
页码：208 / 210
页数：3
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