CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF A BACILLUS-SUBTILIS AND THERMUS-THERMOPHILUS HB8 CHIMERIC 3-ISOPROPYLMALATE DEHYDROGENASE

被引：0

作者：

ONODERA, K ^{[1
]}

MORIYAMA, H ^{[1
]}

TAKENAKA, A ^{[1
]}

TANAKA, N ^{[1
]}

AKUTSU, N ^{[1
]}

MURO, M ^{[1
]}

OSHIMA, T ^{[1
]}

IMADA, K ^{[1
]}

SATO, M ^{[1
]}

KATSUBE, Y ^{[1
]}

机构：

[1] OSAKA UNIV,INST PROT RES,SUITA,OSAKA 565,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1991年 / 109卷 / 01期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A chimeric gene was constructed by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, and expressed in Escherichia coli. The chimeric enzyme was crystallized in a size suitable for X-ray structure analysis. The crystal has a space group of P3(1)21 or P3(2)21, a = b = 77.1-angstrom and c = 158.3-angstrom, which is isomorphous with that of the native enzyme from T. thermophilus.

引用

页码：1 / 2

页数：2

共 50 条

[1] CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF A BACILLUS-SUBTILIS AND THERMUS-THERMOPHILUS HB8 CHIMERIC 3-ISOPROPYLMALATE DEHYDROGENASE AND THERMOSTABLE MUTANTS OF IT
SAKURAI, M
ONODERA, K
MORIYAMA, H
MATSUMOTO, O
TANAKA, N
NUMATA, K
IMADA, K
SATO, M
KATSUBE, Y
OSHIMA, T
JOURNAL OF BIOCHEMISTRY, 1992, 112 (02): : 173 - 174
[2] CRYSTALLIZATION AND PRELIMINARY-X-RAY DATA FOR 3-ISOPROPYLMALATE DEHYDROGENASE OF THERMUS-THERMOPHILUS
KATSUBE, Y
TANAKA, N
TAKENAKA, A
YAMADA, T
OSHIMA, T
JOURNAL OF BIOCHEMISTRY, 1988, 104 (05): : 679 - 680
[3] CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF ISOCITRATE DEHYDROGENASE FROM THERMUS-THERMOPHILUS HB8
OHZEKI, M
YAOI, T
MORIYAMA, H
OSHIMA, T
TANAKA, N
JOURNAL OF BIOCHEMISTRY, 1995, 118 (04): : 679 - 680
[4] COENZYME SPECIFICITY OF 3-ISOPROPYLMALATE DEHYDROGENASE FROM THERMUS-THERMOPHILUS HB8
MIYAZAKI, K
OSHIMA, T
PROTEIN ENGINEERING, 1994, 7 (03): : 401 - 403
[5] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF A NADH OXIDASE FROM THERMUS-THERMOPHILUS HB8
ERDMANN, H
HECHT, HJ
PARK, HJ
SPRINZL, M
SCHOMBURG, D
SCHMID, RD
JOURNAL OF MOLECULAR BIOLOGY, 1993, 230 (03) : 1086 - 1088
[6] 3-DIMENSIONAL STRUCTURES OF CHIMERIC ENZYMES BETWEEN BACILLUS-SUBTILIS AND THERMUS-THERMOPHILUS 3-ISOPROPYLMALATE DEHYDROGENASES
ONODERA, K
SAKURAI, M
MORIYAMA, H
TANAKA, N
NUMATA, K
OSHIMA, T
SATO, M
KATSUBE, Y
PROTEIN ENGINEERING, 1994, 7 (04): : 453 - 459
[7] HIS273 OF 3-ISOPROPYLMALATE DEHYDROGENASE FROM THERMUS-THERMOPHILUS HB8 IS INVOLVED IN THE COENZYME BINDING
YAOI, T
MIYAZAKI, K
OSHIMA, T
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 210 (03) : 733 - 737
[8] Substrate recognition of isocitrate dehydrogenase and 3-isopropylmalate dehydrogenase from Thermus thermophilus HB8
Yaoi, T
Miyazaki, K
Oshima, T
JOURNAL OF BIOCHEMISTRY, 1997, 121 (01): : 77 - 81
[9] THE CRYSTAL-STRUCTURES OF MUTATED 3-ISOPROPYLMALATE DEHYDROGENASE FROM THERMUS-THERMOPHILUS HB8 AND THEIR RELATIONSHIP TO THE THERMOSTABILITY OF THE ENZYME
MORIYAMA, H
ONODERA, K
SAKURAI, M
TANAKA, N
KIRINOKAGAWA, H
OSHIMA, T
KATSUBE, Y
JOURNAL OF BIOCHEMISTRY, 1995, 117 (02): : 408 - 413
[10] Coenzyme activity of NAD analogs for 3-isopropylmalate dehydrogenase from Thermus thermophilus HB8
Chiba, A
Eguchi, T
Oshima, T
Kakinuma, K
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1999, 63 (09) : 1647 - 1649

← 1 2 3 4 5 →