PLANT TISSUE-BASED AMPEROMETRIC ELECTRODE FOR ELIMINATING ASCORBIC-ACID INTERFERENCES

被引：37
作者：
WANG, J
NASER, N
OZSOZ, M
机构：
[1] Department of Chemistry, New Mexico State University, Las Cruces
来源：
ANALYTICA CHIMICA ACTA | 1990年 / 234卷 / 02期
基金：
美国国家卫生研究院;
关键词：
Dopamine; Enzyme electrode; Norepinephrine;
D O I：
10.1016/S0003-2670(00)83572-2
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An application of tissue-based electrodes aimed at eliminating interferences from co-existing electroactive constituents is described. The concept is illustrated using a zucchini-containing carbon paste electrode. The presence of the enzyme ascorbic acid oxidase (AAO) in the zucchini tissue effectively eliminates contributions from ascorbic acid, thus allowing selective measurement of dopamine or norepinephrine. In comparison with analogous enzyme-based AAO electrodes, the tissue "eliminator" electrode offers high biocatalytic stability and activity and extremely low cost. The effects of various experimental variables are studied using pulse voltammetry, chronoamperometry and flow-injection amperometry. The electrode has a useful lifetime of 4 weeks. Simultaneous elimination of uric acid interferences is obtained via the co-immobilization of uricase. Oxygen background currents are eliminated in the presence of ascorbic acid. © 1990.
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页码：315 / 320
页数：6
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