PHOTODYNAMIC EFFECT OF HEMATOPORPHYRIN THROUGHOUT THE CELL-CYCLE OF THE HUMAN CELL LINE NHIK-3025 CULTIVATED INVITRO

Cells from the established cell line NHIK 3025 were synchronized by repeated mitotic selections. Survival of the synchronized cells after treatment with haematoporphyrin and near-UV light was measured by testing the capacity of the cells to form macroscopic colonies.The sensitivity to photodynamic inactivation was small in early G1, late S and G2. The sensitivity increased throughout late G1 and early S to a maximum in mid S. More than a 100-fold variation is found in the survival after 20 min irradiation in the presence of 4 x 10-4M haematoporphyrin. © 1979, The British Empire Cancer Campaign for Research. All rights reserved.