BINDING OF SPIN-LABELED DERIVATIVES OF NAD+ AND ITS STRUCTURAL COMPONENTS TO PIG SKELETAL-MUSCLE LACTATE-DEHYDROGENASE

被引：4
作者：
DEPARADE, MP
TROMMER, WE
机构：
[1] UNIV STUTTGART,INST BIOCHEM,D-7000 STUTTGART 80,FED REP GER
[2] UNIV STUTTGART,INST ORGAN CHEM,D-7000 STUTTGART 80,FED REP GER
[3] UNIV STUTTGART,INST ISOTOPENFORSCH,D-7000 STUTTGART 80,FED REP GER
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 568卷 / 01期
关键词：
(Pig skeletal muscle); Coenzyme binding; Cooperativity; Lactate dehydrogenase; NAD[!sup]+[!/sup; Spin-label;
D O I：
10.1016/0005-2744(79)90284-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The binding of spin-labeled derivatives of NAD+ and its structural components to pig skeletal muscle lactate dehydrogenase (l-lactate:NAD+ oxidoreductase, EC 1.1.1.27) is described. In contrast to results previously obtained with the heart muscle isozyme (Wenzel, H.R., Pfleiderer, G., Trommer, W.E., Paschenda, K. and Redhardt, A. (1976) Biochim. Biophys. Acta 452, 292-301), no significant increase is observed in the binding constant of N6, SL-ADP as compared N6-SL-AMP. This different behavior can be explained by the substitution of glutamine-31 for alanine in the muscle isozyme, which has been proposed to account for the tighter binding of NADH to the heart type. In both isozymes the binding of the spin-labled coenzyme itself is weaker than found for its structural components. © 1979.
引用
收藏
页码：177 / 182
页数：6
相关论文
共 23 条
[1]   MALEIMIDE INACTIVATION OF LACTATE-DEHYDROGENASE ISOENZYMES [J].
ANDERSON, BM ;
VERCELLOTTI, SV ;
FISHER, TL .
BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 350 (01) :135-140
[2]  
Bergmeyer H. U, 1970, METHODEN ENZYMATISCH
[3]  
Berliner Lj, 1976, SEELING SPIN LABELLI
[4]   SIMPLE GRAPHICAL METHOD FOR DETERMINING INHIBITION CONSTANTS OF MIXED, UNCOMPETITIVE AND NON-COMPETITIVE INHIBITORS [J].
CORNISHB.A .
BIOCHEMICAL JOURNAL, 1974, 137 (01) :143-144
[5]  
Dahlquist F W, 1978, Methods Enzymol, V48, P270
[6]   STRUCTURAL ADAPTATIONS OF LACTATE-DEHYDROGENASE ISOZYMES [J].
EVENTOFF, W ;
ROSSMANN, MG ;
TAYLOR, SS ;
TORFF, HJ ;
MEYER, H ;
KEIL, W ;
KILTZ, HH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (07) :2677-2681
[7]   EFFECT OF COMPONENT PARTS OF NICOTINAMIDE ADENINE DINUCLEOTIDE (NAD+) AS INHIBITORS OF LACTIC DEHYDROGENASE [J].
FORLANO, AJ .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1967, 56 (06) :763-&
[8]   STUDIES ON PYROPHOSPHATES .3. NEW METHOD FOR SYNTHESIS OF NUCLEOTIDE COENZYMES BY MEANS OF DI-N-BUTYLPHOSPHINOTHIOYL BROMIDE [J].
FURUSAWA, K ;
SEKINE, M ;
HATA, T .
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1976, (16) :1711-1716
[9]   THERMODYNAMIC STUDIES OF BINARY AND TERNARY COMPLEXES OF PIG-SKELETAL-MUSCLE LACTATE-DEHYDROGENASE [J].
HINZ, HJ ;
SCHMIDT, R ;
SCHEURMANN, W ;
JAENICKE, R .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1977, 80 (02) :543-550
[10]   STUDIES ON AN ENERGY STRUCTURE-FUNCTION RELATIONSHIP OF DEHYDROGENASES .2. CALORIMETRIC INVESTIGATIONS ON INTERACTION OF COENZYME FRAGMENTS WITH PIG SKELETAL-MUSCLE LACTATE-DEHYDROGENASE [J].
HINZ, HJ ;
STEININGER, G ;
SCHMID, F ;
JAENICKE, R .
FEBS LETTERS, 1978, 87 (01) :83-86
123