HIGH-PERFORMANCE SIZE-EXCLUSION CHROMATOGRAPHY OF WOOD HEMICELLULOSES ON A POLY(2-HYDROXYETHYL METHACRYLATE CO-ETHYLENE DIMETHACRYLATE) COLUMN WITH SODIUM-HYDROXIDE SOLUTION AS ELUENT

被引：33

作者：

EREMEEVA, TE

BYKOVA, TO

机构：

[1] Cellulose Laboratory, Institute of Wood Chemistry, Latvian Academy of Sciences, Riga, 226006

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 639卷 / 02期

关键词：

D O I：

10.1016/0021-9673(93)80250-C

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The determination of the molecular mass parameters of wood hemicelluloses by high-performance size-exclusion chromatography (HPSEC) using a poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) (Separon S HEMA 1000) column and 0.5 M NaOH as solvent and eluent is described. It is shown that universal calibration between dextran and xylan is valid. The Mark-Houwink equation for xylan in 0.5 M NaOH was found to be [eta] = 2.67 . 10(-4) M0.73, where M = molecular mass. A direct method of characterization of hemicelluloses extracted with 18% NaOH from different celluloses and pulps by HPSEC is described.

引用

页码：159 / 164

页数：6

共 21 条

[1] POLY-ELECTROLYTES AS SECONDARY CALIBRATION STANDARDS FOR AQUEOUS SIZE-EXCLUSION CHROMATOGRAPHY [J].