CHARACTERIZATION OF TRIACYLGLYCEROLS IN THE SEEDS OF AQUILEGIA-VULGARIS BY CHROMATOGRAPHIC AND MASS-SPECTROMETRIC METHODS

被引：21

作者：

DEMIRBUKER, M

BLOMBERG, LG

OLSSON, NU

BERGQVIST, M

HERSLOF, BG

JACOBS, FA

机构：

[1] UNIV STOCKHOLM,DEPT ANALYT CHEM,S-10691 STOCKHOLM,SWEDEN

[2] KARLSHAMNS LIPIDTEKN AB,S-10465 STOCKHOLM,SWEDEN

[3] SWEDISH PULP & PAPER RES INST,S-11486 STOCKHOLM,SWEDEN

来源：

LIPIDS | 1992年 / 27卷 / 06期

关键词：

D O I：

10.1007/BF02536385

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A combination of analytical techniques is generally necessary to properly characterize complex lipid materials. Chromatographic separation in conjunction with spectroscopic characterization was utilized for the analysis of the triacylglycerols in the seeds of Aquilegia vulgaris. Reversed-phase high-performance liquid chromatography (HPLC), micropacked argentation supercritical fluid chromatography (SFC), and combinations of the two techniques were used. The fatty acid profile was determined by gas chromatography/mass spectrometry of the picolinyl esters and by gas chromatography/flame-ionization detection of the methyl esters. The major components were also identified by direct inlet mass spectrometry. The excellent selectivity of packed fused silica argentation SFC for the separation of triacylglycerols was demonstrated.

引用

页码：436 / 441

页数：6