ALTERED EXPRESSION OF THE CHICKEN HOMEOBOX-CONTAINING GENES GHOX-7 AND GHOX-8 IN THE LIMB BUDS OF LIMBLESS MUTANT CHICK-EMBRYOS

被引:0
作者
COELHO, CND
KRABBENHOFT, KM
UPHOLT, WB
FALLON, JF
KOSHER, RA
机构
[1] UNIV CONNECTICUT,CTR HLTH,SCH MED,DEPT ANAT,FARMINGTON,CT 06030
[2] UNIV CONNECTICUT,CTR HLTH,SCH DENT MED,DEPT BIOSTRUCT & FUNCT,FARMINGTON,CT 06030
[3] UNIV WISCONSIN,DEPT ANAT,MADISON,WI 53706
来源
DEVELOPMENT | 1991年 / 113卷 / 04期
关键词
HOMEOBOX-CONTAINING GENES; LIMB DEVELOPMENT (CHICK); PATTERN FORMATION; APICAL ECTODERMAL RIDGE; LIMBLESS MUTANT CHICK EMBRYOS;
D O I
暂无
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
It has been suggested that the reciprocal expression of the chicken homeobox-containing genes GHox-8 and GHox-7 by the apical ectodermal ridge and subjacent limb mesoderm might be involved in regulating the proximodistal outgrowth of the developing chick limb bud. In the present study the expression of GHox-7 and GHox-8 has been examined by in situ and dot blot hybridization in the developing limb buds of limbless mutant chick embryos. The limb buds of homozygous mutant limbless embryos form at the proper time in development (stage 17/18), but never develop an apical ectodermal ridge, fail to undergo normal elongation, and eventually degenerate. At stage 18, which is shortly following the formation of the limb bud, the expression of GHox-7 is considerably reduced (about 3-fold lower) in the mesoderm of limbless mutant limb buds compared to normal limb bud mesoderm. By stages 20 and 21, as the limb buds of limbless embryos cease outgrowth, GHox-7 expression in limbless mesoderm declines to very low levels, whereas GHox-7 expression increases in the mesoderm of normal limb buds which are undergoing outgrowth. In contrast to GHox-7, expression of GHox-8 in limbless mesoderm at stage 18 is quantitatively similar to its expression in normal limb bud mesoderm, and in limbless and normal mesoderm GHox-8 expression is highly localized in the anterior mesoderm of the limb bud. In normal limb buds, GHox-8 is also expressed in high amounts by the apical ectodermal ridge. However, expression of GHox-8 is severely impaired in the ectoderm of limbless mutant limb buds, which lack an apical ectodermal ridge. The severely impaired expression of GHox-7 in the mesoderm of limbless limb buds suggests that the normal expression of GHox-7 by limb mesoderm during limb bud outgrowth may be regulated by the apical ectodermal ridge, and further suggests the possibility that expression of GHox-7 by subridge mesenchymal cells may be required for normal limb outgrowth to occur. Furthermore, the severely impaired expression of GHox-8 by limbless ectoderm suggests GHox-8 may indeed be involved in ridge function. These observations also raise the interesting possibility that normal GHox-8 expression by the apical ectodermal ridge may be required for normal expression of GHox-7 by the subridge mesenchymal cells.
引用
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页码:1487 / 1493
页数:7
相关论文
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