CELL-CULTURE OF TAXUS AS A SOURCE OF THE ANTINEOPLASTIC DRUG TAXOL AND RELATED TAXANES

被引:127
作者
FETTNETO, AG
DICOSMO, F
REYNOLDS, WF
SAKATA, K
机构
[1] UNIV TORONTO,CTR PLANT BIOTECHNOL,DEPT BOT,25 WILLCOCKS ST,TORONTO M5S 3B2,ON,CANADA
[2] UNIV TORONTO,DEPT CHEM,TORONTO M5S 3B2,ON,CANADA
[3] NIPPON OIL CO LTD,CENT TECH RES LAB,NAKA KU,NAKAGAWA,JAPAN
来源
BIO-TECHNOLOGY | 1992年 / 10卷 / 12期
关键词
D O I
10.1038/nbt1292-1572
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Callus cultures of Taxus cuspidata an Taxus canadensis were induced using different tissue explants including green and red arils, seed contents, young stems and needles. Callus derived from stem segments displayed the best growth in defined media. The culture medium was supplemented with reducing agents and phenolic-binding compounds to inhibit callus darkening and subsequent growth reduction. T. cuspidata explant growth was affected by different concentrations and ratios of 2,4-D and kinetin. Callus tissues of T. cuspidata were extracted for taxol after 2 months in culture and analysed by HPLC. The presence of taxol (0.020 +/- 0.005% of the extracted dry weight) was indicated based on retention time, U.V. spectra, peak purity as assessed by photo-diode array spectroscopy and compared with an authentic taxol standard, as well as by H-1-NMR analysis. Suspension cultures of T. cuspidata were established from the callus cultures, subsequently immobilized onto glass fiber mats, and maintained as immobilized cultures for 6 months. The immobilized cell cultures also produced taxol at levels up to 0.012 +/- 0.007% of the extracted dry weight.
引用
收藏
页码:1572 / 1575
页数:4
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