REGULATION OF TRANSCRIPTION OF THE CEL1 GENE IN AGARICUS-BISPORUS

被引：22

作者：

YAGUE, E ^{[1
]}

WOOD, DA ^{[1
]}

THURSTON, CF ^{[1
]}

机构：

[1] HORT RES INT,LITTLEHAMPTON BN17 6LP,W SUSSEX,ENGLAND

来源：

MOLECULAR MICROBIOLOGY | 1994年 / 12卷 / 01期

关键词：

D O I：

10.1111/j.1365-2958.1994.tb00993.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Northern analysis showed that accumulation of Agaricus bisporus cel1 mRNA was regulated by two independent mechanisms: (i) induction by cellulose; and (ii) repression by glucose and other sugars. Isolated A. bisporus nuclei were transcriptionally active. Nuclei isolated from cellulose-grown mycelium synthesized six times more cel1 mRNA than nuclei from glucose-grown mycelium. The start point of transcription (tsp) was identified by primer extension and S1 nuclease analysis. Putative glucose-, and cAMP-responsive elements as well as regions with homology to promoter regions of other fungal cellulase genes were detected both upstream and downstream from the tsp of the cel1 gene.

引用

页码：41 / 47

页数：7

共 35 条

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