A BASOLATERAL LACTATE H+ COTRANSPORTER IN MADIN-DARBY CANINE KIDNEY (MDCK) CELLS

Monolayers of Madin-Darby Canine Kidney (MDCK) cells grown on permeable filters generated lactate aerobically and accumulated it preferentially in the basolateral compartment, suggesting the presence of a lactate carrier. The mechanism of lactate transport across apical and basolateral membranes was examined by determining intracellular pH (pH(i)) microspectrofluorimetrically after addition of lactate to the extracellular solutions and by measuring uptake of [C-14]lactate. Addition of 20 mM lactate to the apical compartment produced no change in pH(i), whereas lactate added to the basolateral compartment rapidly and reversibly lowered pH(i). Pyruvate produced similar results. Inhibitors of lactate/H+ co-transporters, alpha-cyano-4-hydroxycinnamate (CnCN) and quercetin, partially inhibited the fall in pH(i) produced by basolateral lactate. In contrast, the disulphonic stilbene DIDS (4,4'-di-isothiocyanostilbene-2,2'-disulphonic acid) produced no inhibition at 0.5 mM. Kinetic analysis was performed by applying basolateral lactate at various concentrations and measuring the rate of entry (DELTApH(i)/min) in the presence and absence of CnCN. Lactate flux was shown to occur by both non-ionic diffusion and a alpha-cyano-4-hydroxycinnamate-sensitive component (carrier). The latter has a K(m) of approximately 7 mM for the lactate anion. Propionate, but not formate, lowered pH(i) to the same degree as did equimolar lactate, but the propionate effect was not inhibited by CnCN. Influx of [C-14]lactate was substantially greater across the basolateral membrane than across the apical membrane and occurred in the absence of Na+. We conclude that MDCK cells grown on permeable filters generate lactate aerobically and transport it across the basolateral membrane by way of a lactate/H+ co-transporter.