THE PHOSPHOLIPASE-A2 OF HUMAN SPERMATOZOA - PURIFICATION AND PARTIAL SEQUENCE

被引：27
作者：
LANGLAIS, J
CHAFOULEAS, JG
INGRAHAM, R
VIGNEAULT, N
ROBERTS, KD
机构：
[1] HOP MAISON NEUVE ROSEMONT,RES CTR,5415 BLVD ASSOMPT,MONTREAL H1T 2M4,QUEBEC,CANADA
[2] UNIV MONTREAL,DEPT BIOCHEM,MONTREAL H3C 3J7,QUEBEC,CANADA
[3] INST MED REPROD MONTREAL,MONTREAL,QUEBEC,CANADA
[4] BIOMEGA INC,MONTREAL,QUEBEC,CANADA
[5] BOEHRINGER INGLEHEIM PHARMACEUT INC,RIDGEFIELD,CT
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 01期
基金：
英国医学研究理事会;
关键词：
D O I：
10.1016/S0006-291X(05)80132-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In view of its proposed key role in the acrosome reaction, phospholipase A2 has been isolated and purified from human spermatozoa. Following SDS-PAGE, a single major band was obtained with an estimated molecular mass of 16.7 kDa. Sequence analysis of the N-terminal portion of the molecule revealed the identity of the first 19 amino acids to be YNYQFGLMIVITKGHFAMV. From this partial analysis it is evident that the phospholipase A2 of human spermatozoa represents a new sequence. Of interest is the location of glutamine-4, phenylalanine-5, methionine-8 and isoleucine-9; this sequence appears to be highly conserved throughout evolution. © 1992 Academic Press, Inc.
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页码：208 / 214
页数：7
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