CONSTRUCTION AND EXPRESSION OF A HYBRID PLASMID CONTAINING THE ESCHERICHIA-COLI THRA-GENES AND THRB-GENES

被引：28

作者：

COSSART, P ^{[1
]}

KATINKA, M ^{[1
]}

YANIV, M ^{[1
]}

STGIRONS, I ^{[1
]}

COHEN, GN ^{[1
]}

机构：

[1] INST PASTEUR, DEPT BIOCHIM & GENET MICROBIENNE, F-75724 PARIS, FRANCE

来源：

MOLECULAR AND GENERAL GENETICS | 1979年 / 175卷 / 01期

关键词：

D O I：

10.1007/BF00267853

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro recombination techniques were used to clone the Escherichia coli thrA and thrB structural genes in the plasmid vector pBR322. The chimeric plasmid was analyzed and characterized genetically, by restriction mapping and DNA sequencing. The limited expression of the threonine biosynthetic enzymes in the strain carrying the recombinant plasmid is discussed. © 1979 Springer-Verlag.

引用

页码：39 / 44

页数：6

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