CONSTRUCTION AND EXPRESSION OF A HYBRID PLASMID CONTAINING THE ESCHERICHIA-COLI THRA-GENES AND THRB-GENES

被引：28

作者：

COSSART, P ^{[1
]}

KATINKA, M ^{[1
]}

YANIV, M ^{[1
]}

STGIRONS, I ^{[1
]}

COHEN, GN ^{[1
]}

机构：

[1] INST PASTEUR, DEPT BIOCHIM & GENET MICROBIENNE, F-75724 PARIS, FRANCE

来源：

MOLECULAR AND GENERAL GENETICS | 1979年 / 175卷 / 01期

关键词：

D O I：

10.1007/BF00267853

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro recombination techniques were used to clone the Escherichia coli thrA and thrB structural genes in the plasmid vector pBR322. The chimeric plasmid was analyzed and characterized genetically, by restriction mapping and DNA sequencing. The limited expression of the threonine biosynthetic enzymes in the strain carrying the recombinant plasmid is discussed. © 1979 Springer-Verlag.

引用

页码：39 / 44

页数：6

共 40 条

[1] MAPPING DNA FRAGMENTS PRODUCED BY CLEAVAGE OF LAMBDA DNA WITH ENDONUCLEASE RI [J].

ALLET, B ;

JEPPESEN, PG ;

KATAGIRI, KJ ;

DELIUS, H .

NATURE, 1973, 241 (5385) :120-123

[2] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].

BOLIVAR, F ;

RODRIGUEZ, RL ;

GREENE, PJ ;

BETLACH, MC ;

HEYNEKER, HL ;

BOYER, HW ;

CROSA, JH ;

FALKOW, S .

GENE, 1977, 2 (02) :95-113

[3]

BRILEY PA, 1978, J BIOL CHEM, V253, P8867

[4] HOMOSERINE KINASE FROM ESCHERICHIA-COLI-K12 [J].

BURR, B ;

WALKER, J ;

TRUFFABACHI, P ;

COHEN, GN .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1976, 62 (03) :519-526

[5] NATURE OF COL E1 PLASMID REPLICATION IN ESCHERICHIA-COLI IN PRESENCE OF CHLORAMPHENICOL [J].

CLEWELL, DB .

JOURNAL OF BACTERIOLOGY, 1972, 110 (02) :667-&

[6] EFFECT OF GROWTH CONDITIONS ON FORMATION OF RELAXATION COMPLEX OF SUPERCOILED COLE1 DEOXYRIBONUCLEIC ACID AND PROTEIN IN ESCHERICHIA-COLI [J].

CLEWELL, DB ;

HELINSKI, DR .

JOURNAL OF BACTERIOLOGY, 1972, 110 (03) :1135-&

[7]

Cohen G. N., 1969, CURR TOP CELL REGUL, V1, P183

[8]

COHEN N, 1956, ANN I PASTEUR PARIS, V91, P693

[9] PRIMARY STRUCTURE OF ESCHERICHIA-COLI-K12 ASPARTOKINASE I-HOMOSERINE DEHYDROGENASE-I - ISOLATION AND CHARACTERIZATION OF PEPTIDES PRODUCED BY CYANOGEN-BROMIDE [J].

COSSARTGHEERBRANT, P ;

SIBILLIWEILL, L ;

BRILEY, PA ;

CHALVIGNAC, MA ;

LEBRAS, G ;

COHEN, GN .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 535 (02) :206-215

[10] REVISED STRUCTURE OF ASPARTOKINASE I-HOMOSERINE DEHYDROGENASE I OF ESCHERICHIA-COLI K12 - EVIDENCE FOR 4 IDENTICAL SUBUNITS [J].

FALCOZKELLY, F ;

TRUFFABA.P ;

VERON, M ;

SAARI, J ;

JANIN, J ;

COHEN, GN .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1972, 28 (04) :507-+

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