TLC-LSIMS OF NEOGLYCOLIPIDS OF GLYCOSAMINOGLYCAN DISACCHARIDES AND OF OXYMERCURATION CLEAVAGE PRODUCTS OF HEPARIN FRAGMENTS THAT CONTAIN UNSATURATED URONIC-ACID

被引：14
作者：
CHAI, WG [1 ]
ROSANKIEWICZ, JR [1 ]
LAWSON, AM [1 ]
机构：
[1] NORTHWICK PK HOSP & CLIN RES CTR,MRC,GLYCOSCI LAB,MASS SPECTROMETRY GRP,HARROW HA1 3UJ,MIDDX,ENGLAND
来源：
CARBOHYDRATE RESEARCH | 1995年 / 269卷 / 01期
关键词：
GLYCOSAMINOGLYCANS; OLIGOSACCHARIDES; OXYMERCURATION; NEOGLYCOLIPIDS;
D O I：
10.1016/0008-6215(94)00350-O
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Heparin and chondroitin sulfate disaccharides have been investigated by high-performance (HP) TLC and liquid secondary-ion mass spectrometry (LSIMS) after conversion to neoglycolipid derivatives by reductive-amination with an aminolipid (dihexadecyl phosphatidylethanol amine, DHPE). Mobility on HPTLC was largely determined by the number of sulfate groups present, but was also influenced by the position of sulfate, monosaccharide composition and linkage. The mass spectra acquired directly from the TLC plate provided quasimolecular and fragment ions from which composition, including sulfate content, and sequence information was obtained at high sensitivity. Lipid DHPE conjugation and TLC-LSIMS were performed to analyse products of the oxymercuration reaction used to cleave unsaturated uronic acid (Delta UA) residues from glycosaminoglycan (GAG) fragments produced by enzymatic degradation with glycan lyases. Previously the identification of the product from Delta UA and the integrity of the remaining structures
引用
收藏
页码：111 / 124
页数：14
相关论文
共 23 条
[1]  
Kjellen, Lindahl, Annu. Rev. Biochem., 60, pp. 443-475, (1991)
[2]  
Hardingham, Fosang, FASEB J., 6, pp. 861-870, (1992)
[3]  
Lindahl, Backstrom, Thunberg, J. Biol. Chem., 258, pp. 9826-9830, (1983)
[4]  
Atha, Stephens, Rosenberg, Proc. Natl. Acad. Sci. U.S.A., 81, pp. 1030-1034, (1984)
[5]  
Hase, Ikenaka, Matsushima, Biochem. Biophys. Res. Commun., 85, pp. 257-263, (1978)
[6]  
Dell, Adv. Carbohydr. Chem. Biochem., 45, pp. 19-72, (1987)
[7]  
Webb, Jiang, Gillece Castro, Tarentino, Plummer, Byrd, Fisher, Burlingame, Anal. Biochem., 169, pp. 337-349, (1988)
[8]  
Lawson, Chai, Cashmore, Stoll, Hounsell, Feizi, Carbohydr. Res., 200, pp. 47-57, (1990)
[9]  
Casu, Heparin, pp. 25-49, (1989)
[10]  
Chai, Cashmore, Carruthers, Stoll, Lawson, Biol. Mass. Spectrom., 20, pp. 169-178, (1991)
123