EPSTEIN-BARR-VIRUS BHRF1 GENE BUT NOT THE CELLULAR PROTOONCOGENE BCL-2 IS EXPRESSED IN ATAXIA-TELANGIECTASIA LYMPHOBLASTOID LINES

被引：4

作者：

BECKER, Y

TABOR, E

ASHER, Y

机构：

[1] Department of Molecular Virology, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem

来源：

VIRUS GENES | 1991年 / 5卷 / 01期

关键词：

ATAXIA-TELANGIECTASIA; LYMPHOBLASTOID CELL LINES EPSTEIN-BARR VIRUS; EBV BHRF1 ORF; BCL-2; PROTOONCOGENE;

D O I：

10.1007/BF00571729

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The expression of both the Epstein-Barr virus (EBV) ORF BHRF1 and the cellular protooncogene bcl-2 was studied in EBV-transformed lymphoblastoid B cells from patients with the human genetic disorder ataxia-telangiectasia (A-T). Using the Northern blot technique, it was found that the pattern of transcription of the BHRF1 gene in A-T lymphoblastoids resembled that in EBV-transformed normal lymphoblastoid lines and Burkitt lymphoma (BL) lymphocytes. However, the 1.5-kb mature BHRF1 mRNA species present in normal lymphoblastoid cells and in BL cells was not found in the A-T lymphoblastoid cell lines. Treatment of the A-T lymphoblastoid lines with phorbol ester caused changes in the pattern of the synthesis and quantity of BHRF1-related RNA transcripts. The bcl-2 protooncogene probe did not detect bcl-2-related mRNA in the A-T lymphoblastoid lines.

引用

页码：33 / 45

页数：13

共 44 条

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