GENERATION AND ANALYSIS OF NONHOMOLOGOUS RNA-RNA RECOMBINANTS IN BROME MOSAIC-VIRUS - SEQUENCE COMPLEMENTARITIES AT CROSSOVER SITES

被引：52

作者：

BUJARSKI, JJ ^{[1
]}

DZIANOTT, AM ^{[1
]}

机构：

[1] NO ILLINOIS UNIV,DEPT BIOL SCI,DE KALB,IL 60115

来源：

JOURNAL OF VIROLOGY | 1991年 / 65卷 / 08期

关键词：

D O I：

10.1128/JVI.65.8.4153-4159.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

All three single-stranded RNAs of the brome mosaic virus (BMV) genome contain a highly conserved, 193-base 3' noncoding region. To study the recombination between individual BMV RNA components, barley plants were infected with a mixture of in vitro-transcribed wild-type BMV RNAs 1 and 2 and an RNA3 mutant that carried a deletion near the 3' end. This generated a population of both homologous and nonhomologous 3' recombinant BMV RNA3 variants. Sequencing revealed that these recombinants were derived by either single or double crossovers with BMV RNA1 or RNA2. The primary sequences at recombinant junctions did not show any similarity. However, they could be aligned to form double-stranded heteroduplexes. This suggested that local hybridizations among BMV RNAs may support intermolecular exchanges.

引用

收藏

页码：4153 / 4159

页数：7

相关论文

共 37 条

[1] NEAR IDENTITY OF 3' RNA SECONDARY STRUCTURE IN BROMOVIRUSES AND CUCUMBER MOSAIC-VIRUS [J].

AHLQUIST, P ;

DASGUPTA, R ;

KAESBERG, P .

CELL, 1981, 23 (01) :183-189

[2] LOCALIZATION OF THE REPLICASE RECOGNITION SITE WITHIN BROME MOSAIC-VIRUS RNA BY HYBRID-ARRESTED RNA-SYNTHESIS [J].

AHLQUIST, P ;

BUJARSKI, JJ ;

KAESBERG, P ;

HALL, TC .

PLANT MOLECULAR BIOLOGY, 1984, 3 (01) :37-44

[3] REGENERATION OF A FUNCTIONAL RNA VIRUS GENOME BY RECOMBINATION BETWEEN DELETION MUTANTS AND REQUIREMENT FOR COWPEA CHLOROTIC MOTTLE VIRUS 3A AND COAT GENES FOR SYSTEMIC INFECTION [J].

ALLISON, R ;

THOMPSON, C ;

AHLQUIST, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (05) :1820-1824

[4] AN IN VITRO SYSTEM FOR THE LEADER-PRIMED TRANSCRIPTION OF CORONAVIRUS MRNAS [J].

BAKER, SC ;

LAI, MMC .

EMBO JOURNAL, 1990, 9 (12) :4173-4179

[5] A CLUSTERING OF RNA RECOMBINATION SITES ADJACENT TO A HYPERVARIABLE REGION OF THE PEPLOMER GENE OF MURINE CORONAVIRUS [J].

BANNER, LR ;

KECK, JG ;

LAI, MMC .

VIROLOGY, 1990, 175 (02) :548-555

[6] SIMILARITY IN STRUCTURE AND FUNCTION OF 3'-TERMINAL REGION OF 4 BROME MOSAIC VIRAL RNAS [J].

BASTIN, M ;

DASGUPTA, R ;

HALL, TC ;

KAESBERG, P .

JOURNAL OF MOLECULAR BIOLOGY, 1976, 103 (04) :737-745

[7] SHORTENED FORMS OF BEET NECROTIC YELLOW VEIN VIRUS RNA-3 AND RNA-4 - INTERNAL DELETIONS AND A SUBGENOMIC RNA [J].

BOUZOUBAA, S ;

NIESBACHKLOSGEN, U ;

JUPIN, I ;

GUILLEY, H ;

RICHARDS, K ;

JONARD, G .

JOURNAL OF GENERAL VIROLOGY, 1991, 72 :259-266

[8] MODULATION OF REPLICATION, AMINOACYLATION AND ADENYLATION INVITRO AND INFECTIVITY INVIVO OF BMV RNAS CONTAINING DELETIONS WITHIN THE MULTIFUNCTIONAL 3' END [J].

BUJARSKI, JJ ;

AHLQUIST, P ;

HALL, TC ;

DREHER, TW ;

KAESBERG, P .

EMBO JOURNAL, 1986, 5 (08) :1769-1774

[9] GENETIC-RECOMBINATION BETWEEN RNA COMPONENTS OF A MULTIPARTITE PLANT-VIRUS [J].

BUJARSKI, JJ ;

KAESBERG, P .

NATURE, 1986, 321 (6069) :528-531

[10] DELETIONS IN THE 3'-TERMINAL TRANSFER RNA-LIKE STRUCTURE OF BROME MOSAIC-VIRUS RNA DIFFERENTIALLY AFFECT AMINOACYLATION AND REPLICATION INVITRO [J].

BUJARSKI, JJ ;

DREHER, TW ;

HALL, TC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (17) :5636-5640

← 1 2 3 4 →