SODIUM-SELECTIVE LIQUID ION-EXCHANGER MICRO-ELECTRODES FOR INTRACELLULAR MEASUREMENTS

被引：71
作者：
ODOHERTY, J
GARCIADIAZ, JF
ARMSTRONG, WM
机构：
来源：
SCIENCE | 1979年 / 203卷 / 4387期
关键词：
D O I：
10.1126/science.424756
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The sodium-selective ligand 1,1,1-tris[11-(21-oxa- 41-oxo-51-aza-51-methyl) dodecanyl]propane dissolved in 3-nitro-o-xylene containing a small amount of the lipophilic anion tetrachlorophenyl borate was used as a liquid ion-exchanger in sodium-selective microelectrodes. The microelectrodes gave rapid, stable responses that were linear functions of the logarithm of sodium activity. They were tested under conditions approximating those to be expected in the cell interior, and the results indicated that they can be used to measure intracellular sodium activity without significant interference from intracellular potassium. Copyright © 1979 AAAS.
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页码：1349 / 1351
页数：3
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