SOLID-STATE NMR STRUCTURAL STUDIES OF PEPTIDES AND PROTEINS IN MEMBRANES

Solid-state NMR spectroscopy is emerging as a new approach in the structural investigations of peptides and proteins in membrane bilayers. Orientational parameters obtained from samples aligned on glass plates have been used to determine their secondary structures and orientations in the bilayer. They have also been used in the determination of the high resolution structure of gramicidin. Additional structural information, distance measurements between pairs of different nuclei and between pairs of like nuclei has been obtained on peptides in bilayers using magic angle spinning methods.