DIRECT PCR DETECTION OF FOOT-AND-MOUTH-DISEASE VIRUS

被引：38
作者：
RODRIGUEZ, A
NUNEZ, JI
NOLASCO, G
PONZ, F
SOBRINO, F
DEBLAS, C
机构：
[1] INIA,CTR INVEST SANIDAD ANIM,E-28130 VALDEOLMOS,SPAIN
[2] INST NACL INVEST AGR,CIT,DEPT PROTECC VEGETAL,E-28080 MADRID,SPAIN
来源：
JOURNAL OF VIROLOGICAL METHODS | 1994年 / 47卷 / 03期
关键词：
FMDV DIAGNOSIS; DIRECT RT-PCR; VIRUS DETECTION;
D O I：
10.1016/0166-0934(94)90030-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A PCR assay for the detection and characterization of foot-and-mouth disease virus was developed. The procedure allows RT-PCR amplification following direct adsorption of viral suspensions to microtiter plates, avoiding previous steps of phenol-extraction or heating. Using this procedure, FMDV-specific (based on 3D gene sequences), as well as serotype-specific (based on VP1 gene sequences) amplification were achieved for viral samples of serotypes A, O and C, either from cell culture supernatants or from lesions of infected animals. The assay allowed detection of around 15 PFU, being 500-fold more sensitive than a conventional indirect ELISA. This new method constitutes a simple, rapid and efficient alternative for the diagnosis and characterization of FMDV by PCR.
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页码：345 / 349
页数：5
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