PRODUCTION OF SPECIFIC ANTIBODY AGAINST L-DOPA AND ITS ULTRASTRUCTURAL-LOCALIZATION OF IMMUNOREACTIVITY IN THE HOUSE-SHREW (SUNCUS-MURINUS) LATERAL HABENULAR NUCLEUS

被引：30
作者：
KARASAWA, N
ISOMURA, G
NAGATSU, I
机构：
[1] Department of Anatomy, School of Medicine, Fujita Health University, Aichi
来源：
NEUROSCIENCE LETTERS | 1992年 / 143卷 / 1-2期
关键词：
L-3,4-DIHYDROXYPHENYLALANINE; TYROSINE HYDROXYLASE; AROMATIC L-AMINO ACID DECARBOXYLASE; DOPAMINE; CATECHOLAMINE; IMMUNOHISTOCHEMISTRY; LATERAL HABENULAR NUCLEUS; HOUSE-SHREW;
D O I：
10.1016/0304-3940(92)90280-K
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
An antiserum was raised against L-DOPA bound to bovine serum albumin, purified by affinity chromatography, and its specificities were verified by immunoblotting and enzyme-linked immunosorbent assays. The antiserum did not cross-react with dopamine (DA), tyrosine, tyramine, octopamine, norepinephrine or epinephrine. Immunocytochemical studies using the PAP method revealed that tyrosine hydroxylase- and L-DOPA positive but aromatic L-amino acid decarboxylase- and DA-negative neurons were present in the lateral habenular nucleus of the house-shrew (Suncus murinus) brain. Ultrastructurally L-DOPA immunoreactive products were localized in the cytoplasmic matrix and terminals with vesicles.
引用
收藏
页码：267 / 270
页数：4
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