CRYSTALLIZATION STUDIES ON HIV-1 REVERSE-TRANSCRIPTASE

被引：2
|
作者：
LLOYD, LF [1 ]
BRICK, P [1 ]
BLOW, DM [1 ]
LOU, MZ [1 ]
机构：
[1] ACAD SINICA,INST BIOPHYS,BEIJING 100080,PEOPLES R CHINA
来源：
JOURNAL OF CRYSTAL GROWTH | 1992年 / 122卷 / 1-4期
关键词：
D O I：
10.1016/0022-0248(92)90269-O
中图分类号：
O7 [晶体学];
学科分类号：
0702 ; 070205 ; 0703 ; 080501 ;
摘要：
HIV-1 reverse transcriptase has been crystallized in a variety of forms. Various ligands used for co-crystallization are described and the results presented. All of these crystals showed disorder when examined in the X-ray beam. The best diffraction currently achieved has been approximately 7 angstrom. The possible reasons for crystal disorder are discussed. An example of another protein, carboxypeptidase G2, Which initially yielded non-diffracting crystals, is used to illustrate the value of applying random or incomplete factorial screens to sample wider parameter space for conditions to grow well-ordered crystals.
引用
收藏
页码：355 / 359
页数：5
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