CLONING AND SEQUENCE-ANALYSIS OF AN EXTREMELY HOMOGENEOUS TANDEMLY REPEATED DNA IN THE GRASSHOPPER EYPREPOCNEMIS-PLORANS

被引：24
作者：
LOPEZLEON, MD
VAZQUEZ, P
HEWITT, GM
CAMACHO, JPM
机构：
[1] UNIV GRANADA,FAC CIENCIAS,DEPT GENET,E-18071 GRANADA,SPAIN
[2] UNIV E ANGLIA,SCH BIOL SCI,NORWICH NR4 7TJ,NORFOLK,ENGLAND
来源：
HEREDITY | 1995年 / 75卷
关键词：
B CHROMOSOMES; DNA SEQUENCING; HETEROCHROMATIN; REPETITIVE DNA; SATELLITE DNA;
D O I：
10.1038/hdy.1995.148
中图分类号：
Q14 [生态学（生物生态学）];
学科分类号：
071012 ; 0713 ;
摘要：
Digestion of total nuclear DNA of the grasshopper Eyprepocnemis plorans with seven different restriction endonucleases (REs), and subsequent agarose gel electrophoresis, has shown the presence of highly repetitive DNA yielding the typical ladder-like banding pattern. The most clear pattern was produced by DraI, the monomer being some 180 bp. This repeat unit was subsequently cloned and sequenced. Bidirectional sequencing of five randomly chosen clones showed exactly the same nucleotides in all 180 positions. The possible explanations for such an extreme homogeneity of this tandem repeat are discussed in the light of current hypotheses on repetitive DNA function and molecular drive mechanisms.
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页码：370 / 375
页数：6
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