REGULATION OF RAG-2 PROTEIN EXPRESSION IN AVIAN THYMOCYTES

被引:12
|
作者
FERGUSON, SE
ACCAVITTI, MA
WANG, DD
CHEN, CL
THOMPSON, CB
机构
[1] UNIV CHICAGO, GWEN KNAPP CTR LUPUS & IMMUNOL RES, CHICAGO, IL 60637 USA
[2] UNIV CHICAGO, DEPT MED, CHICAGO, IL 60637 USA
[3] UNIV CHICAGO, DEPT MOLEC GENET & CELL BIOL, CHICAGO, IL 60637 USA
[4] UNIV CHICAGO, HOWARD HUGHES MED INST, CHICAGO, IL 60637 USA
[5] UNIV ALABAMA, DEPT MICROBIOL, BIRMINGHAM, AL 35294 USA
[6] UNIV ALABAMA, DIV DEV & CLIN IMMUNOL, BIRMINGHAM, AL 35294 USA
[7] UNIV ALABAMA, DEPT MED, BIRMINGHAM, AL 35294 USA
关键词
D O I
10.1128/MCB.14.11.7298
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The recombinase-activating genes, RAG-1 and RAG-2, have been shown to be necessary to initiate the process of V(D)J recombination during the ontogeny of lymphocytes. While much is known about the end products of this rearrangement process, little is known about the function or regulation of the components of the recombinase system. To this end, we have generated a monoclonal antibody to the chicken RAG-2 protein. Chicken thymocytes were found to express high levels of RAG-2, part of which is phosphorylated. Within thymocytes, RAG-2 is expressed primarily within the nucleus. RAG-2 protein levels are high in the CD4(-) CD8(-) CD4(+) CD8(+) immature thymocytes but absent at the single-positive CD4(+) CD8(-) or CD4(-) CD8(+) stage of thymocyte development. Mitogenic stimulation of thymocytes with phorbol myristate acetate and ionomycin results in down-regulation of RAG-2 expression. Consistent with these data, in vivo levels of RAG-2 are markedly lower in proliferating thymocytes than in smaller, G(0)/G(1) cells. Down-regulation of RAG-2 expression appears to occur before cells enter S phase, suggesting that RAG-2 function may be limited to noncycling cells.
引用
收藏
页码:7298 / 7305
页数:8
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